RNA silencing publications using FANA oligos

Days of using transfection reagents are over. FANA oligos are easily taken up by the cells without transfection or delivery agents

N. Souleimanian, G.F. Deleavey, H. Soifer, S. Wang, K. Tiemann, M.J. Damha and C.A. Stein (2012), Antisense 2′-deoxy-2′-fluoroarabinonucleic acids (2′F-ANAs) antisense oligonucleotides: in vitro gymnotic silencers of gene expression whose potency is enhanced by fatty acids, Molecular Therapy-Nucleic Acids, Sep 18; 1:e43; doi: 10.1038/mtna.2012.35.

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FANA oligos are resistant to degradation by serum and cellular nucleases and exhibit greater potency and stability

A. Dudley, M. Sater, P.U. Le, G. Trinh, M.S. Sadr, J. Bergeron, G.F. Deleavey, B. Bedell, M.J. Damha and K. Petrecca (2014) DRR regulates AKT activation to drive brain cancer invasion, Oncogene, 33:4952-60
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N. Ferrari, D. Bergeron, A.-L. Tedeschi, M.M. Mangos, L. Paquet, P.M. Renzi and M.J. Damha (2006) Characterization of antisense oligonucleotides comprised of 2′-deoxy-2′-fluoro-b-D-arabinonucleic acid (FANA): specificity, potency and duration of activity, Annals of the New York Academy of Sciences, 1082: 91–102.
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A. Kalota, L. Karabon, C Swider, E. Viazovkina, M. Elzagheid, M.J. Damha and A. Gewirtz (2006) 2′-Deoxy-2′-fluoro-b-D-arabinonucleic acid (2′F-ANA), modified oligonucleotides (ON) effect highly efficient, and persistent, gene silencing, Nucleic Acids Research, 34: 451-461.
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FANA sugar modification significantly enhances chemical stability

J.K. Watts, A. Katolik, J. Viladoms and M.J. Damha (2009), Studies on the hydrolytic stability of 2′-fluoroarabinonucleic acid (2′F-ANA), Organic & Biomolecular Chemistry, 7:1904 – 1910.
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FANA sugar modification significantly enhances intracellular stability

A. Kalota, L. Karabon, C Swider, E. Viazovkina, M. Elzagheid, M.J. Damha and A. Gewirtz (2006) 2′-Deoxy-2′-fluoro-b-D-arabinonucleic acid (2′F-ANA), modified oligonucleotides (ON) effect highly efficient, and persistent, gene silencing, Nucleic Acids Research, 34: 451-461.

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N. Ferrari, D. Bergeron, A.-L. Tedeschi, M.M. Mangos, L. Paquet, P.M. Renzi and M.J. Damha (2006) Characterization of antisense oligonucleotides comprised of 2′-deoxy-2′-fluoro-b-D-arabinonucleic acid (FANA): specificity, potency and duration of activity, Annals of the New York Academy of Sciences, 1082: 91–102.

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